The effects of anionic electrolytes and human serum albumin on the LCST of poly(N-isopropylacrylamide)-based temperature-responsive copolymers.

摘要: Poly(N-isopropylacrylamide) (PNIPAm) is one of the most widely studied temperature-responsive polymers among those that have been applied to biomaterials science and technology. Here, we investigated importance interactions between PNIPAm-based copolymers biological factors. The effects a series major anionic electrolytes in environments human serum albumin (HSA) on lower critical solution temperature (LCST) homo-PNIPAm PNIPAm were studied, using either hydrophobic monomer or cationic monomer. We synthesized P(NIPAm-co-BMA3%) with butyl methacrylate (BMA) as P(NIPAm-co-DMAPAm2%) N,N-dimethylaminopropyl acrylamide (DMAPAm) LCST decreased increasing salt concentrations, anions each polymer corresponded Hofmeister series. was greatly affected by compared P(NIPAm-co-BMA3%). While not HSA, non-linearly HSA concentrations. These due electrostatic positively charged chains negatively well stabilization aggregations HSA. Under physiological buffer conditions, significantly concentration. results indicated depending types used for applications, it necessary take into account effect media while designing polymers.