FTIR Study of Horseradish Peroxidase in Reverse Micelles
作者： Jianbo Chen , Chungu Xia , Jianzhong Niu , Shu-ben Li
关键词: Aqueous solution 、 Micelle 、 Horseradish peroxidase 、 Chromatography 、 Fourier transform infrared spectroscopy 、 Nuclear chemistry 、 Chemistry 、 Pulmonary surfactant 、 Protein secondary structure 、 Bromide 、 Sodium 、 Biophysics 、 Cell biology 、 Biochemistry 、 Molecular biology
摘要: Fourier transform infrared (FTIR) method was used to study the secondary structures of horseradish peroxidase (HRP) in aqueous solution and reverse micelles for first time. Results indicated that structure HRP sodium bis(2-ethylhexy)sulfosuccinate (AOT) close solution. In cetyltrimethylammonium bromide (CTAB) dodecylfate (SDS) position some bands changed. had a relationship with surfactant species micelles. Among three types micelles, system AOT probably most beneficial reaction media HRP.
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