PLFA analyses of microbial communities associated with PAH-contaminated riverbank sediment.

作者: Brenda Pratt , Roland Riesen , Carl G. Johnston

DOI: 10.1007/S00248-012-0060-8

关键词: PopulationAquatic ecosystemMicrobial population biologyMicrobial ecologyCommunity structureEcologyBiologyBioremediationAnaerobic bacteriaSediment

摘要: Sediment contaminated with polycyclic aromatic hydrocarbons (PAHs) is widely distributed in aquatic ecosystems. The microbial community structure of riverbank PAH-contaminated sediments was investigated using phospholipid-derived fatty acid (PLFA) analysis. Surface and subsurface sediment collected from a highly site an uncontaminated along the Mahoning River, OH. PAH concentrations, physical characteristics, other parameters (biomass as phospholipid phosphate (PLP) activity) were also measured. PAHs detected all samples but only quantifiable (250 μg/g g−1) sediment. Subsurface both locations showed very similar PLP values distribution PLFAs, 27–37 % being composed sulfate reducing anaerobic bacteria. Principal components analysis indicated no correlation between contamination PLFA diversity. Although measurements bacterial communities did not reflect environmental differences among sites, highest measured activity (reduction 1,200 nmol INT g−1 h−1), likely population adapted to pollutants, rates that are much higher than many soil systems. These data warrant further investigation into at genetic level indicate potential for bioremediation by indigenous microbes.

参考文章(53)
J. Robie Vestal, David C. White, Lipid analysis in microbial ecology: quantitative approaches to the study of microbial communities. BioScience. ,vol. 39, pp. 535- 541 ,(1989) , 10.2307/1310976
Sheridan Kidd Haack, Helen Garchow, David A. Odelson, Larry J. Forney, Michael J. Klug, Accuracy, reproducibility, and interpretation of Fatty Acid methyl ester profiles of model bacterial communities. Applied and Environmental Microbiology. ,vol. 60, pp. 2483- 2493 ,(1994) , 10.1128/AEM.60.7.2483-2493.1994
Donald E. Langworthy, Raymond D. Stapleton, Gary S. Sayler, Robert H. Findlay, Genotypic and Phenotypic Responses of a Riverine Microbial Community to Polycyclic Aromatic Hydrocarbon Contamination Applied and Environmental Microbiology. ,vol. 64, pp. 3422- 3428 ,(1998) , 10.1128/AEM.64.9.3422-3428.1998
L.D. Rasmussen, F. Ekelund, L.H. Hansen, S.J. Sørensen, K. Johnsen, Group-Specific PCR Primers to Amplify 24S a-Subunit rRNA Genes from Kinetoplastida (Protozoa) Used in Denaturing Gradient Gel Electrophoresis Microbial Ecology. ,vol. 42, pp. 109- 115 ,(2001) , 10.1007/S002480000120
Metin Duran, Berat Z. Haznedaroğlu, Daniel H. Zitomer, Microbial source tracking using host specific FAME profiles of fecal coliforms. Water Research. ,vol. 40, pp. 67- 74 ,(2006) , 10.1016/J.WATRES.2005.10.019
Janet Joynt, Marianne Bischoff, Ron Turco, Allan Konopka, Cindy H Nakatsu, None, Microbial community analysis of soils contaminated with lead, chromium and petroleum hydrocarbons. Microbial Ecology. ,vol. 51, pp. 209- 219 ,(2006) , 10.1007/S00248-005-0205-0
Robert G. Björk, Mats P. Björkman, Mats X. Andersson, Leif Klemedtsson, Temporal variation in soil microbial communities in Alpine tundra Soil Biology & Biochemistry. ,vol. 40, pp. 266- 268 ,(2008) , 10.1016/J.SOILBIO.2007.07.017
S. Jeffery, J.A. Harris, R.J. Rickson, K. Ritz, Microbial community phenotypic profiles change markedly with depth within the first centimetre of the arable soil surface Soil Biology & Biochemistry. ,vol. 39, pp. 1226- 1229 ,(2007) , 10.1016/J.SOILBIO.2006.12.023
Robert M. Dunn, Juha Mikola, Roland Bol, Richard D. Bardgett, Influence of microbial activity on plant-microbial competition for organic and inorganic nitrogen Plant and Soil. ,vol. 289, pp. 321- 334 ,(2006) , 10.1007/S11104-006-9142-Z
D.E. Langworthy, R.D. Stapleton, G.S. Sayler, R.H. Findlay, Lipid analysis of the response of a sedimentary microbial community to polycyclic aromatic hydrocarbons. Microbial Ecology. ,vol. 43, pp. 189- 198 ,(2002) , 10.1007/S00248-001-1040-6