Cell cycle analysis of p53-induced cell death in murine erythroleukemia cells.

摘要: A temperature-sensitive mutant of murine p53 (p53Val-135) was transfected by electroporation into erythroleukemia cells (DP16-1) lacking endogenous expression p53. While the grew normally in presence (37.5 degrees C), wild-type (32.5 C) associated with a rapid loss cell viability. Genomic DNA extracted at 32.5 C seen to be fragmented characteristic ladder consistent death due apoptosis. Following synchronization density arrest, released G1 were found lose viability more rapidly than did randomly growing cultures. release G1, became irreversibly committed after 4 h C. Commitment correlated first appearance DNA. Synchronized allowed pass out prior being placed continued cycle until subsequently arrested G1; occurred following arrest. In contrast cultured for prolonged periods during S phase and G2/M, then returned 37.5 C, not become death. arrest utilizing either mimosine or isoleucine deprivation, does lead Upon transfer these synchronized populations quickly lost Cells that kept (G0) much slower rate G1. Taken together, results indicate induces this effect occurs predominantly actively cycling cells.