Alkaline Phosphatase-Catalyzed Silver Deposition for Electrochemical Detection

摘要: Alkaline phosphatase (AP) is one of the most used enzymatic labels for development ELISAs, immunosensors, DNA hybridization assays, etc. This enzyme catalyzes dephosphorylation a substrate into detectable product usually quantified by optical or electrochemical measurements. work based on (3-indoxyl phosphate) that produces compound able to reduce silver ions in solution metallic deposit, which localized where label AP attached. The deposited electrochemically stripped and measured anodic stripping voltammetry. Its application an genosensor streptavidin-modified screen-printed carbon electrodes detection virulence nucleic acid determinants autolysin gene, exclusively present genome human pathogen Streptococcus pneumoniae, described. Compared with direct voltammetric indigo carmine, voltammetry 14-fold more sensitive.