Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach.
作者： Rita C. Alves , Filipa B. Pimentel , Henri P. A. Nouws , Wagner Correr , María Begoña González-García
DOI: 10.1007/S00216-015-8879-8
关键词: Allergen 、 Biosensor 、 Chromatography 、 Antibodies monoclonal 、 Linear range 、 Voltammetry 、 Immunoassay 、 Conductometry 、 Chemistry 、 Electrochemical detection
摘要: A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction detected through the electrochemical of enzymatically deposited silver. immunosensor presented linear range between 1 and 100 ng/ml, as well high precision (inter-day RSD ≤9.8%) accuracy (recoveries ≥96.7%). quantification limits 0.27 0.88 respectively. It possible detect small levels complex matrices.
springer.com
core.ac.uk
sci-hub.se 参考文章(24)
Graciela Martínez-Paredes, María Begoña González-García, Agustín Costa-García, In situ electrochemical generation of gold nanostructured screen-printed carbon electrodes. Application to the detection of lead underpotential deposition Electrochimica Acta. ,vol. 54, pp. 4801- 4808 ,(2009) , 10.1016/J.ELECTACTA.2009.03.085
Yin Huang, Melissa C. Bell, Ian I. Suni, Impedance biosensor for peanut protein Ara h 1. Analytical Chemistry. ,vol. 80, pp. 9157- 9161 ,(2008) , 10.1021/AC801048G
Rita C. Alves, Filipa B. Pimentel, Henri P.A. Nouws, Raquel C.B. Marques, María Begoña González-García, M. Beatriz P.P. Oliveira, Cristina Delerue-Matos, Detection of Ara h 1 (a major peanut allergen) in food using an electrochemical gold nanoparticle-coated screen-printed immunosensor. Biosensors and Bioelectronics. ,vol. 64, pp. 19- 24 ,(2015) , 10.1016/J.BIOS.2014.08.026
K Govatsi, A Chrissanthopoulos, V Dracopoulos, S N Yannopoulos, The influence of Au film thickness and annealing conditions on the VLS-assisted growth of ZnO nanostructures. Nanotechnology. ,vol. 25, pp. 215601- ,(2014) , 10.1088/0957-4484/25/21/215601
Rita C. Alves, M. Fátima Barroso, María Begoña González-García, M. Beatriz P.P. Oliveira, Cristina Delerue-Matos, New Trends in Food Allergens Detection: Toward Biosensing Strategies Critical Reviews in Food Science and Nutrition. ,vol. 56, pp. 2304- 2319 ,(2016) , 10.1080/10408398.2013.831026
Maria Pele, Marcel Brohée, Elke Anklam, Arjon J. Van Hengel, Peanut and hazelnut traces in cookies and chocolates: relationship between analytical results and declaration of food allergens on product labels. Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment. ,vol. 24, pp. 1334- 1344 ,(2007) , 10.1080/02652030701458113
Pablo Fanjul-Bolado, David Hernández-Santos, María Begoña González-García, Agustín Costa-García, Alkaline Phosphatase-Catalyzed Silver Deposition for Electrochemical Detection Analytical Chemistry. ,vol. 79, pp. 5272- 5277 ,(2007) , 10.1021/AC070624O
Linda Monaci, Angelo Visconti, Mass spectrometry-based proteomics methods for analysis of food allergens TrAC Trends in Analytical Chemistry. ,vol. 28, pp. 581- 591 ,(2009) , 10.1016/J.TRAC.2009.02.013
J. Pollet, F. Delport, K.P.F. Janssen, D.T. Tran, J. Wouters, T. Verbiest, J. Lammertyn, Fast and accurate peanut allergen detection with nanobead enhanced optical fiber SPR biosensor. Talanta. ,vol. 83, pp. 1436- 1441 ,(2011) , 10.1016/J.TALANTA.2010.11.032
Marat Khodoun, Richard Strait, Tatyana Orekov, Simon Hogan, Hajime Karasuyama, R Herbert De'Broski, Jörg Köhl, Fred D Finkelman, None, Peanuts can contribute to anaphylactic shock by activating complement Journal of Allergy and Clinical Immunology. ,vol. 123, pp. 342- 351 ,(2009) , 10.1016/J.JACI.2008.11.004