Detection of Ara h 1 (a major peanut allergen) in food using an electrochemical gold nanoparticle-coated screen-printed immunosensor.
作者： Rita C. Alves , Filipa B. Pimentel , Henri P.A. Nouws , Raquel C.B. Marques , María Begoña González-García
DOI: 10.1016/J.BIOS.2014.08.026
关键词:
摘要: Abstract A gold nanoparticle-coated screen-printed carbon electrode was used as the transducer in development of an electrochemical immunosensor for Ara h 1 (a major peanut allergen) detection food samples. Gold nanoparticles (average diameter=32 nm) were electrochemically generated on surface electrodes. Two monoclonal antibodies a sandwich-type immunoassay and antibody–antigen interaction detected through stripping analysis enzymatically (using alkaline phosphatase) deposited silver. The total time optimized 3 h 50 min. developed allowed quantification between 12.6 2000 ng/ml, with limit 3.8 ng/ml, provided precise (RSD 96.6%) results. successfully applied to complex matrices (cookies chocolate), being able detect samples containing 0.1% peanut.
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