Same-vessel enzymatic saccharification and fermentation of organosolv/H2O2 pretreated oil palm (Elaeis guineensis Jacq.) fronds for bioethanol production: Optimization of process parameters

摘要: Abstract Based on optimized pretreatment process, oil palm fronds (OPFs) were sequentially pretreated with 1.4% (w/v) aq. NaOH in 80% ethanol ultrasound assistance (at 75 °C for 30 min) and 3% (v/v) H 2 O . Using the Box–Behnken design (BBD) of response surface methodology (RSM), bioethanol production from sono-assisted organosolv/H OPFs using same-vessel enzymatic saccharification fermentation (SVSF) where both hydrolysis processes carried out one vessel simultaneously. Throughout SVSF incubation time enzyme loading kept at 72 h 15 filter paper unit (FPU)/g substrate respectively. The other parameters which affect yield such as temperature ( X 1 : 30–50 °C), solid 5.0–10.0% w/v), yeast concentration 3 5.0–20 g/l) pH 4 4.0–7.0) optimized. Well fitted regression equations R  > 0.97) obtained able to predict reliable optimum yield. predicted (i.e., 20.61 g/l) 84.60%) attained 36.94 °C (∼37 °C), 7.57% w/v (∼8.0% 13.97 g/l (∼14.0 g/l) 5.29 (∼5.30). Validated results indicated a maximum 21.96 g/l 84.65% respectively, closer responses. conditions, highest productivity 0.76 g/l/h was observed 12 h process.