Studies on the mechanism of the photosensitized inactivation of E. coli and reactivation phenomenon.

摘要: In order to find a more satisfactory interpretation of the phenomenon photosensitized inactivation bacteria, studies were performed under various experimental conditions on methylene blue and E. coli. summary findings are as follow:— 1. The dye is absorbed by bacteria according Langmuir isotherm can be removed ionic substitutions; binding predominantly ionic; dye-bacteria complex produces new absorption peak in 610 mµ wave length region, action spectrum corresponds spectral complex. 2. There an optimum concentration range for inactivation. 3. Photosensitized take place both frozen liquid states presence oxygen essential process. 4. Hydrogen peroxide, formed reoxidation reduced blue, does not inactivate bacteria. 5. Following inactivation, coli lose their ability reduce hydrogen donors, suggesting that enzymes involved 6. Bacteria inactivated photosensitization reactivated prolonged storage after irradiation; recovery rate increases with increasing temperature (maximum 37°), also influenced donors. view collected data, basic reaction mechanisms analyzed first step seems excitation dye-bacteria, or complex, photon which activated such state, molecular capable producing oxidizing reaction, results Some aspects detailed reactions taking at cell surface discussed.