Purification and identification of a polysaccharide from medicinal mushroomAmauroderma rudewith immunomodulatory activity and inhibitory effect on tumor growth

摘要: // Honghui Pan 1 , Yuanyuan Han Jiguo Huang Xiongtao Yu Chunwei Jiao 2 Xiaobing Yang Preet Dhaliwal 3, 4 Yizhen Xie Burton B. Guangdong Institute of Microbiology, State Key Laboratory Applied Microbiology Southern China, Provincial Microbial Culture Collection and Application, Open Guangzhou, China Yuewei Edible Fungi Technology Co. Ltd., 3 Sunnybrook Research Institute, Health Sciences Centre, Toronto, Canada Department Medicine Pathobiology, University Correspondence to: Xie, e-mail: 13622216490@126.com Yang, byang@sri.utoronto.ca Keywords: medicinal mushroom, herbal medicine, tumor growth, cytokine, amauroderma Received: April 28, 2015      Accepted: June 15, Published: 27, 2015 ABSTRACT Medicinal mushrooms in recent years have been the subject many experiments searching for anticancer properties. We previously screened thirteen their potential inhibiting found that water extract Amauroderma rude exerted highest activity. Previous studies shown polysaccharides contained were responsible This study was designed to explore effects on immune regulation growth. Using crude extract, vitro showed capacities spleen lymphocytes, macrophages, natural killer cells all increased. In vivo increased macrophage metabolism, lymphocyte proliferation, antibody production. addition, partially purified product stimulated secretion cytokines . Overall, decreased growth rates. Lastly, active compound identified as polysaccharide F212. Most importantly, had activity increasing proliferation. summary, this molecule may serve a lead drug development.