Self-Assembling Protein Microarrays
作者： Niroshan Ramachandran , Eugenie Hainsworth , Bhupinder Bhullar , Samuel Eisenstein , Benjamin Rosen
关键词: Protein microarray 、 Computational biology 、 Single-stranded binding protein 、 Plasma protein binding 、 Protein structure 、 Protein biosynthesis 、 FLAG-tag 、 Genetics 、 DNA replication factor CDT1 、 Biology 、 DNA replication 、 Multidisciplinary
摘要: Protein microarrays provide a powerful tool for the study of protein function. However, they are not widely used, in part because challenges producing proteins to spot on arrays. We generated by printing complementary DNAs onto glass slides and then translating target with mammalian reticulocyte lysate. Epitope tags fused allowed them be immobilized situ. This obviated need purify proteins, avoided stability problems during storage, captured sufficient functional studies. used technology map pairwise interactions among 29 human DNA replication initiation recapitulate regulation Cdt1 binding select its geminin-binding domain.
sciencemag.org
harvard.edu
elsevier.com参考文章(26)
Dolores J. Cahill, Eckhard Nordhoff, Protein arrays and their role in proteomics. Advances in Biochemical Engineering \/ Biotechnology. ,vol. 83, pp. 177- 187 ,(2003) , 10.1007/3-540-36459-5_7
Kevin D. Lustig, P.Todd Stukenberg, Thomas J. McGarry, Randall W. King, Vincent L. Cryns, Paul E. Mead, Leonard I. Zon, Junying Yuan, Marc W. Kirschner, Small pool expression screening: identification of genes involved in cell cycle control, apoptosis, and early development. Methods in Enzymology. ,vol. 283, pp. 83- 99 ,(1997) , 10.1016/S0076-6879(97)83009-1
Albertha J.M. Walhout, Gary F. Temple, Michael A. Brasch, James L. Hartley, Monique A. Lorson, Sander van den Heuvel, Marc Vidal, GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods in Enzymology. ,vol. 328, pp. 575- 592 ,(2000) , 10.1016/S0076-6879(00)28419-X
Stuart L. Schreiber, Gavin MacBeath, Printing proteins as microarrays for high-throughput function determination. Science. ,vol. 289, pp. 1760- 1763 ,(2000) , 10.1126/SCIENCE.289.5485.1760
Heng Zhu, Metin Bilgin, Rhonda Bangham, David Hall, Antonio Casamayor, Paul Bertone, Ning Lan, Ronald Jansen, Scott Bidlingmaier, Thomas Houfek, Tom Mitchell, Perry Miller, Ralph A Dean, Mark Gerstein, Michael Snyder, None, Global Analysis of Protein Activities Using Proteome Chips Science. ,vol. 293, pp. 2101- 2105 ,(2001) , 10.1126/SCIENCE.1062191
Lee Zou, Bruce Stillman, Formation of a Preinitiation Complex by S-phase Cyclin CDK-Dependent Loading of Cdc45p onto Chromatin Science. ,vol. 280, pp. 593- 596 ,(1998) , 10.1126/SCIENCE.280.5363.593
James A Wohlschlegel, Suman K Dhar, Tatyana A Prokhorova, Anindya Dutta, Johannes C Walter, Xenopus Mcm10 Binds to Origins of DNA Replication after Mcm2-7 and Stimulates Origin Binding of Cdc45 Molecular Cell. ,vol. 9, pp. 233- 240 ,(2002) , 10.1016/S1097-2765(02)00456-2
Kenneth McClain, Zeev Estrov, Uma Raju, Patrick K. Kelley, Bharat B. Aggarwal, Epstein–Barr Virus EBNA-2 Gene Expression Enhances Lymphotoxin Production by B Lymphocytes Methods. ,vol. 11, pp. 83- 87 ,(1997) , 10.1006/METH.1996.0391
Margot Kneissl, Vera Pütter, Aladar A Szalay, Friedrich Grummt, Interaction and Assembly of Murine Pre-replicative Complex Proteins in Yeast and Mouse Cells Journal of Molecular Biology. ,vol. 327, pp. 111- 128 ,(2003) , 10.1016/S0022-2836(03)00079-2
Suman Kumar Dhar, Laurie Delmolino, Anindya Dutta, Architecture of the human origin recognition complex. Journal of Biological Chemistry. ,vol. 276, pp. 29067- 29071 ,(2001) , 10.1074/JBC.M103078200