Formation of a Preinitiation Complex by S-phase Cyclin CDK-Dependent Loading of Cdc45p onto Chromatin
作者： Lee Zou , Bruce Stillman
DOI: 10.1126/SCIENCE.280.5363.593
关键词: Chromatin remodeling 、 Origin recognition complex 、 Cell biology 、 Pre-replication complex 、 Scaffold/matrix attachment region 、 Molecular biology 、 Minichromosome maintenance 、 S phase 、 Cyclin-dependent kinase 、 Chromatin 、 Biology 、 Multidisciplinary
摘要: Cdc45p, a protein essential for initiation of DNA replication, associates with chromatin after "start" in late G1 and during the S phase cell cycle. Binding Cdc45p to depends on Clb-Cdc28 kinase activity as well functional Cdc6p Mcm2p, which suggests that prereplication complex activation S-phase cyclin-dependent kinases (CDKs). As indicated by timing CDK dependence, binding is crucial commitment replication. During phase, physically interacts minichromosome maintenance (MCM) proteins chromatin; however, dissociation from slower than MCMs, indicates are released different mechanisms.
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