Method for preparing bioreactor

摘要: The present invention is a method for purifying an enzyme, particularly acylase, comprising causing selective aggregation and precipitation of contaminant deacetylase, by the use surfactant, cationic surfactant. also regenerating immobilized enzyme carrier, synthetic adsorbent or ion exchange resin, allowing protease to act on remove from said being prepared binding with carrier optionally crosslinking enzymes agent after binding. Particularly, above-mentioned regeneration method, wherein has fine pores, cephalosporin C acylase. According purification invention, undesirable objective which cannot be removed conventional can selectively separated removed. Therefore, inventive useful production standard product having higher purity. In addition, based degradation extremely efficiently, so that repeated recycled realized, beneficial in terms environmental economical aspects.