Purifying cephalosporin C acylase and regenerating a carrier immobilizing cephalosporin C acylase

摘要: An enzyme in a mixture containing the and contaminant is purified by selectively aggregating precipitating with surfactant. A carrier an immobilized regenerated using protease to remove from carrier. Cephalosporin C acylase of deacetylase adding solution benzylated cationic or methylated surfactant amount 0.1 0.6% mixture. The may be alkyl(palm)dimethylbenzyl ammonium chloride. cephalosporin contacting alkaline acidic protease. being bound optionally crosslinked. have pores 100 nm less diameter.