Total internal reflection fluorescence microscopy.
作者： Daniel Axelrod
DOI: 10.1016/S0091-679X(08)60982-6
关键词: Fluorescence 、 Total internal reflection 、 Fluorescence microscope 、 Optics 、 Microscopy 、 Total internal reflection fluorescence microscope 、 Fluorescence recovery after photobleaching 、 Optical sectioning 、 Biology 、 Confocal microscopy
摘要: Total internal reflection fluorescence (TIRF) microscopy provides a means to selectively excite fluorophores in an aqueous or cellular environment that is very near solid surface (within ≤100 nm: less than one-fifth the thickness of confocal section) without exciting from regions further surface. Fluorescence excitation by this thin zone electromagnetic energy (called evanescent field) results images with low background fluorescence, virtually no out-of-focus and minimal exposure cells light any other planes sample. Figure 21-1 shows example TIRF on intact living culture compared standard epifluorescence. The above features have led numerous applications, including following:
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