作者: William M. Coenen , Lyle T. Sinor , William L. Bayer , Malcolm L. Beck , Frederick V. Plapp
DOI:
关键词: Antibody screening 、 Molecular biology 、 Membrane 、 Biology 、 Blood grouping 、 Antigen 、 Red blood cell 、 Antibody 、 Antibody identification 、 Proteolytic enzymes
摘要: A method of forward blood grouping is presented wherein known antibodies are attached to a solid surface and the red cells for immunological reaction activated with proteolytic enzyme. reverse procedure utilizes synthetic or purified antigens which directly surface. The then contacted an unknown component permit undergo previously antigens. solution used as indicator. performing major crossmatch anti-human immunoglobulin that by two components antigen-antibody interaction antibodies. antibody sensitized will immunologically adhere phase. In alternative procedure, binder attach cell membranes from donor serum recipient allowed immune these on Antibody screening identification carried out attaching antigen carrying extent specific adhered present. Red particles coated immunoglobulins indicator mechanism.