Method for drying mammalian cells for use in solid phase immunoassays and articles incorporating same

作者: Ralph A. Eatz , Lyle T. Sinor

DOI:

关键词: Salt (chemistry)Aqueous solutionDecantationDisaccharideChemistrySodiumChromatographyMonolayerCyclitolMonosaccharide

摘要: A method for drying mammalian cells, such as erythrocytes, lumphocytes, leukocytes and platelets onto a solid-phase support use in immunoassays through of solution an article prepared by method. The comprises immobilizing monolayer cells the non-­covalent binding. This is accomplished staining with organic dye having net positive charge which permits non-covalent binding carry negative to support. These are dried or fixed addition aqueous monosaccharide, disaccharide, trisaccharide cyclitol salt. preferred monosaccharide D-(-)glucose salt sodium chloride. 1.0M dextrose 154mM contacted immobilized immediately decanted any excess water removed solid-­phase sealed pouch dessicant material 3-8 days at between 2-8°C. amount time required complete 7

参考文章(6)
William M. Coenen, Lyle T. Sinor, William L. Bayer, Malcolm L. Beck, Frederick V. Plapp, Testing for a blood group immunological reaction ,(1983)
Christine V. Benedict, Paul T. Picciano, Bioadhesives for cell and tissue adhesion ,(1987)
Bruce Joseph Roser, Protection of proteins and the like ,(1986)
Martin L. Golick, Michael L. Rice, Stephen F. Healy, Control slide for immunoassay kit and method of making same ,(1987)