SVP-like MADS-box protein from Carya cathayensis forms higher-order complexes.

摘要: To properly regulate plant flowering time and construct floral pattern, MADS-domain containing transcription factors must form multimers including homo- hetero-dimers. They are also active in forming hetero-higher-order complexes with three to five different molecules. However, it is not well known if a MADS-box protein can homo-higher-order complex. In this study biochemical approach utilized provide insight into the complex formation for an SVP-like cloned from hickory. The results indicated that heterogeneous higher-order peak population over 20 monomers. Y2H verified homo-complex yeast cells. Western blot of hickory bud sample revealed exists polymers native. Deletion assays flexible C-terminal residues mainly responsible polymer heterogeneity. Current direct evidences be high order complex, much higher than quartermeric polymer. Analysis suggests subset may able self-assemble large complexes, thereby differentiate one subfamily other structural manner. Present result valuable supplement action mechanism proteins development.