Spectrophotometric assay of thiols
作者： Peter C. Jocelyn
DOI: 10.1016/0076-6879(87)43013-9
关键词: Oxidizing agent 、 Halide 、 Organic chemistry 、 Trinitrobenzenesulfonic Acid 、 Chemistry 、 Absorbance 、 Stoichiometry 、 Mixed disulfides 、 Reagent 、 Thiol
摘要: Publisher Summary This chapter summarizes the chief methods in use for thiols general. These exploit either of two principal properties SH group—namely, its capacity oxidation or substitution. Oxidizing agents may be analytical value if they do not oxidize further than disulfides (that is, to sulfinic sulfonic acids) 75 Se-labeled and also give absorbance changes sufficient magnitude. The describes aromatic that fulfill these requirements best and, are reagents first choice. Aromatic dominate field because easily reduced by thiol-disulfide exchange, equilibrium over-whelmingly favoring formation thiol. Mixed stable intermediate products with protein thiols, but nonprotein themselves rapidly reduced. In case, overall stoichiometry is one thiol generated each biological originally present. Various organic oxidants described lodosobenzoic acid, diphenylpicrylphenylhydrazine, benzofuroxan, quinines, 4, 4’-bisdimethylaminodiphenylcarbinol, trinitrobenzenesulfonic acid. inorganic described. explores substitution exploited spectrophotometric assay includes mercurials, nitrous acids, maleimides, halides, others.
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