Maternal cholestasis does not affect the ontogenic pattern of expression of the Na+/Taurocholate cotransporting polypeptide (ntcp) in the fetal and neonatal rat liver

摘要: To assess the effects of cholestasis during pregnancy on fetal and neonatal mRNA expression, protein mass, function Na+/taurocholate cotransporting polypeptide (Ntcp), common bile duct ligation (BDL) was performed in pregnant rats day 14 (maternal [MC] group), livers were harvested at days 20 21 life, as well 4, 7, 14, 21, 28 after birth. Sham-operated their litters used controls. Ntcp steady-state levels, determined by Northern blotting, immunoblotting, taurocholate (TC) transport studies isolated short-term cultured hepatocytes, respectively. In addition, mass organic anion transporting (Oatp1), another sinusoidal acid transporter, studied 4 weeks age. The majority cholestatic (94%) able to carry term. Body liver weights offspring from MC group lower than those sham-operated animals all postnatal time points. unaffected MC. ontogenic pattern expression identical controls with detection life. There a significant increase postnatally, reaching adult levels 7 Protein Oatp1 similar control groups conclusion, maternal obstructive last third does not affect fetal/neonatal basolateral transporters, Oatp1. This suggests that impaired excretion described this experimental model is related altered uptake acids affected neonate.