In Silico and Biochemical Characterization of Lysozyme-Like Proteins in the Rat.

摘要: Background Spermatogenesis and sperm maturation in the male reproductive tract is dictated by a variety of proteins secreted testis epididymis. Though proteome these tissues known, functional role many remains uncharacterized. In this study, we characterize rat Lysozyme-like (Lyzl) genes proteins. Methods In silico tools were used to predict primary, secondary tertiary structures. Reverse transcription PCR, immunofluorescence immunoblotting determine expression pattern. Lysozyme like enzyme activity was assessed standard assays. Results Six Lyzl namely Lyzl1, Lyzl3, Lyzl4, Lyzl5, Lyzl6 Lyzl7 found be highly conserved among vertebrates with higher homology mouse counterparts than human counterparts. All LYZL contained characteristic 4 disulfide bridges similar c-type lysozyme. Only 1 6, active site amino acids Molecular modeling studies indicated that exhibit strikingly three-dimensional structures themselves. The structure analysis recombinant presence α-helix, β-sheet random coil α-helix being majority. Docking peptidoglycan binding nature proteins. mRNA transcripts (Lyzl1, Lyzl7) are predominantly expressed testes though some them other tract. Their androgen independent. localized germinal epithelium on spermatozoa. Recombinant LYZL1 6 possessed muramidase, isopeptidase antibacterial activities. mechanism action LYZL6 involved bacterial membrane damage leakage cellular contents. possess ability, whereas LYZL3, LYZL4 LYZL5 hyaluronan ability suggesting possible divergence LYZL7 free radical scavenging property, they may act as antioxidants. Conclusion The divergent properties indicate have function, innate immunity physiological process well.