Visco-hyperelastic characterization of the mechanical properties of human fallopian tube tissue using atomic force microscopy

摘要: Abstract Human ovum cells sense the physical stimuli of their environment and regulate behavior based on these stimuli. The fallopian tube tissue, as substrate which is fertilized, has major interactions with makes its mechanical properties great significance. In in-vitro fertilization (IVF), however, are fertilized non-biomechanical substrates that more than five orders magnitude harder tube. This huge difference in natural IVF can have negative result development embryo, success process long-term health effects children born using IVF. As a first step toward replicating biologically relevant for process, this study characterizes visco-hyperelastic Nano-indentation experiments rates ranging from 0.5 to 10 μm/s were conducted ampullary-isthmic junction human tissue Atomic-Force Microscopy. Apparent Young's modulus found Hertzian contact model showed strong correlation indentation rate it increased 26.86 110.69 kPa when 10 μm/s. dependency well high nonlinearity observed data, investigated by model, Ogden strain energy function. By developing an optimization reverse finite element method parameters captured. final results show tangent shear 11.5 kPa relaxation time 34.5 ms tissue.