Frontal Affinity Chromatography with Mass Spectrometry Detection for Probing Molecular Interactions. A New Mechanistic Approach for the Development of Detection & Identification and Medical Countermeasures Against CB Agents

摘要: Abstract : Frontal affinity chromatography with mass spectrometry detection (FAC-MS) is a powerful technique for the study of molecular interactions between receptors (proteins, antibodies, enzymes) and ligands (small molecules, antigens, drug compounds). Such are fundamental to biochemical pathways, from protein cascade involved in disease state, mechanisms, high-throughput screening identification optimization lead compounds discovery. FAC-MS able locate characterize key recognitions complex biological systems that otherwise "hidden" current technologies. Thus, this cutting-edge can steer biotechnological research through areas eliminate many years "hit-and-miss" experimentation. It will allow rational design development areas, such as bio-detection identification, medical countermeasure, which currently interest CF DRDC. amenable large collections simultaneously. Currently, no other alternative method provide unbiased, direct measures binding rapid easy perform manner FAC-MS. This article outlines plan adapting industry (Protana Inc.) DRDC Suffield defense incorporating toxin research, discovery, recombinant antigen-antibody proteomics.