Structure and activity of lipid membrane biosensor surfaces studied with atomic force microscopy and a resonant mirror.
作者： Michael I Fisher , Torbjörn Tjärnhage
DOI: 10.1016/S0956-5663(00)00105-6
关键词:
摘要: Three variants of the liposome fusion (coalescence) method to produce supported lipid bilayers, containing ganglioside GM1 on silicon nitride surfaces, were studied. The first procedure involved attachment and liposomes DMPC, a small amount biotinylated (Biotin-LC-DPPE) streptavidin coated surface. Direct composed mixture DPPC, DPPG, DPPE, cholesterol surface second variant. final utilised type liposomes, fused onto layer with exposed hydrophobic tails. methods produced similar layers, but different ways binding cholera toxin B-subunit (CTB) towards these sensor surfaces was measured in resonant mirror biosensor instrument activity longer-term stability layers examined. prepared also imaged by atomic force microscopy (AFM) liquid characterise topography layers. efficiency CTB discussed terms fluidity roughness.
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sci-hub.se 参考文章(25)
R. R. C. New, Liposomes : a practical approach IRL Press : Oxford University Press. ,(1990)
Robert B. Gennis, Biomembranes : molecular structure and function ,(1989)
P.B. Contino, C.A. Hasselbacher, J.B. Ross, Y. Nemerson, Use of an oriented transmembrane protein to probe the assembly of a supported phospholipid bilayer Biophysical Journal. ,vol. 67, pp. 1113- 1116 ,(1994) , 10.1016/S0006-3495(94)80577-6
Samuel Terrettaz, Thierry Stora, Claus Duschl, Horst Vogel, Protein binding to supported lipid membranes: investigation of the cholera toxin-ganglioside interaction by simultaneous impedance spectroscopy and surface plasmon resonance Langmuir. ,vol. 9, pp. 1361- 1369 ,(1993) , 10.1021/LA00029A033
F. Jähnig, P. Nollert, H. Kiefer, Lipid vesicle adsorption versus formation of planar bilayers on solid surfaces Biophysical Journal. ,vol. 69, pp. 1447- 1455 ,(1995) , 10.1016/S0006-3495(95)80014-7
J. Hoh, R. Lal, S. John, J. Revel, M. Arnsdorf, Atomic force microscopy and dissection of gap junctions Science. ,vol. 253, pp. 1405- 1408 ,(1991) , 10.1126/SCIENCE.1910206
Per-Åke Ohlsson, Torbjörn Tjärnhage, Erik Herbai, Stefan Löfås, Gertrud Puu, Liposome and proteoliposome fusion onto solid substrates, studied using atomic force microscopy, quartz crystal microbalance and surface plasmon resonance. Biological activities of incorporated components Bioelectrochemistry and Bioenergetics. ,vol. 38, pp. 137- 148 ,(1995) , 10.1016/0302-4598(95)01821-U
Xuedong Song, John Nolan, Basil I. Swanson, Optical Signal Transduction Triggered by Protein−Ligand Binding: Detection of Toxins Using Multivalent Binding Journal of the American Chemical Society. ,vol. 120, pp. 4873- 4874 ,(1998) , 10.1021/JA980246S
C.A. Keller, B. Kasemo, Surface specific kinetics of lipid vesicle adsorption measured with a quartz crystal microbalance. Biophysical Journal. ,vol. 75, pp. 1397- 1402 ,(1998) , 10.1016/S0006-3495(98)74057-3
Peter H. Fishman, Role of membrane gangliosides in the binding and action of bacterial toxins The Journal of Membrane Biology. ,vol. 69, pp. 85- 97 ,(1982) , 10.1007/BF01872268