作者: Michael C. Heinrich , Ilya Levental , Hannah Gelman , Paul A. Janmey , Tobias Baumgart
DOI: 10.1021/JP7116246
关键词: Lipid raft 、 Thermal fluctuations 、 Dipole 、 Chemical physics 、 Analytical chemistry 、 Chemistry 、 Monolayer 、 Membrane 、 Critical exponent 、 Surface pressure 、 Yield (chemistry)
摘要: The potential physiological relevance of liquid−liquid phase separation in lipid membranes to the formation and stability “lipid rafts” cellular plasma has prompted extensive investigation physical chemistry underlying these phenomena. In this contribution, line tension (γ) dipole density differences (µ) between demixed fluid phases monolayers comprised dimyristoylphosphatidylcholine (DMPC) dihydrocholesterol (DChol) were investigated by measuring two-dimensional thermal fluctuations domain boundaries visualized inclusion a fluorescent tracer lipid. These parameters are essential determinants stability, their quantification will yield an increased understanding processes responsible for aspects lateral separation. Employing data set, surface pressure dependence γ µ was determined at three different monolayer compositions (30%, 35%, 40% DChol). Both found decrease ...