φX-174 Bacteriophage Structural Mutants Which Affect Deoxyribonucleic Acid Synthesis

摘要: Abstract Seven cistrons in X-174 were identified and one particular was studied intensively: cistron A, which is assigned a protein the mature phage. Amber mutants this synthesize new deoxyribonucleic acid (DNA) form addition to circular phage DNA upon infection of restrictive host. This linear, non-infectious, single-stranded; it formed from strand replicative DNA. These produce two different defective particles One particle contains but not infectious; other similar 70S found wild-type lysates. The mutant A gene product acts independently normal mixed host with an any or wild type.