Most tumors in transgenic mice with human c-Ha-ras gene contained somatically activated transgenes.

摘要: Two independent transgenic mouse lines carrying human hybrid c-Ha-ras genes with their own promoter region encoding prototype products, were established. In these lines, about 50% of offspring had tumors within 18 months. The developed in restricted tissues and 60% affected mice angiosarcomas. transgenes expressed both the all normal tissues. However, somatic mutational activation was detected only tumors. point mutation at 61st codon, from CAG(Gln) to CTG(Leu), angiosarcomas (22/22), some lung adenocarcinomas (3/11) Harderian gland (4/7) lines. other 12th codon GGC(Gly) GTC(Val) two four skin papillomas. No mutations on codons mice. Nontransgenic littermates no all. From results, it strongly suggested that do not develop by expression transgenes, definite certain cell types may be a causative event tumorigenesis