Metabolomics and lipidomics using traveling-wave ion mobility mass spectrometry.

摘要: Metabolomics and lipidomics aim to profile the wide range of metabolites lipids that are present in biological samples. Recently, ion mobility spectrometry (IMS) has been used support metabolomics applications facilitate separation identification complex mixtures analytes. IMS is a gas-phase electrophoretic technique enables ions gas phase according their charge, shape size. Occurring within milliseconds, compatible with modern mass (MS) operating microsecond scan speeds. Thus, time required for acquiring data does not affect overall run traditional liquid chromatography (LC)-MS-based experiments. The addition conventional LC-MS-based workflows shown enhance peak capacity, spectral clarity fragmentation specificity. Moreover, by enabling determination collision cross-section (CCS) value-a parameter related ions-IMS can improve accuracy metabolite identification. In this protocol, we describe how integrate traveling-wave (TWIMS) into metabolomic lipidomic workflows. particular, procedures following: tuning calibrating SYNAPT High-Definition MS (HDMS) System (Waters) specifically applications; extracting polar from brain samples; setting up appropriate chromatographic conditions; simultaneously m/z, retention CCS values each analyte; processing analyzing using dedicated software solutions, such as Progenesis QI (Nonlinear Dynamics); and, finally, performing lipid databases TWIMS-derived information.