Human interferon-alpha increases the cytotoxic effect of CD56+cord blood-derived cytokine-induced killer cells on human B-acute lymphoblastic leukemia cell lines

摘要: Background aims. Cytokine-induced killer (CIK) cells may represent a promising immunotherapy for the treatment of children with relapsing B-lineage acute lymphoblastic leukemia (B-ALL) following hematopoietic stem cell transplantation (HSCT). Therefore, we investigated possibility combining adoptive CIK and human interferon-alpha (hIFN- α ) in order to potentiate cytotoxicity against B-ALL. Methods. Cord bloodderived (CB-CIK) were differentiated, stimulated phosphate-buffered saline (PBS) or hIFN- , tested cytotoxic activity. We anti-leukemic graft-versus-host disease (GvHD) effects CB-CIK xenograft NOD/SCID/ γ c � (NSG) mouse model. Results. Bulk showed very moderate activity while subpopulation CD56 signifi cant B-ALL cells. cantly augmented vitro induced signal transducer activator transcription-1 (STAT1) phosphorylation. In addition, could delay mortality vivo this effect was enhanced by ( P 0.022). Furthermore, unlike CB mononuclear peripheral blood (PBMC), cells, alone caused either no GvHD mild GvHD, respectively, when injected into sublethally irradiated NSG mice. Conclusions. are effective agents lines possess that is potentiated an model vivo. These pre-clinical data support testing immunotherapeutic approach clinic