The long non-coding RNA H19-derived miR-675 modulates human gastric cancer cell proliferation by targeting tumor suppressor RUNX1.
作者： Ming Zhuang , Wen Gao , Jing Xu , Ping Wang , Yongqian Shu
DOI: 10.1016/J.BBRC.2013.12.126
关键词: Long non-coding RNA 、 Blot 、 Carcinogenesis 、 Transcription (biology) 、 Downregulation and upregulation 、 Molecular biology 、 RUNX1 、 Cell growth 、 Cancer research 、 Biology 、 Suppressor
摘要: The lncRNA H19 has been recently shown to be upregulated and play important roles in gastric cancer tumorigenesis. However, the precise molecular mechanism of its mature product miR-675 carcinogenesis remains unclear. In this study, we found that was positively expressed with a pivotal mediator H19-induced cell growth promotion. Subsequently, tumor suppressor Runt Domain Transcription Factor1 (RUNX1) confirmed direct target using luciferase reporter assay Western blotting analyses. A series rescue assays indicated RUNX1 mediated H19/miR-67-induced phenotypic changes. Moreover, inverse relationship between expression H19/miR-675 also revealed tissues lines. Taken together, our study demonstrated novel pathway H19/miR-675/RUNX1 regulates development may serve as potential for therapy.
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