Nuclear proteomic changes linked to soybean rust resistance

摘要: Soybean rust, caused by the fungus Phakopsora pachyrhizi, is an emerging threat to US soybean crop. In effort identify proteins that contribute disease resistance in we compared a susceptible Williams 82 cultivar resistant inbred isoline harboring Rpp1 gene (R-gene). Approximately 4975 from nuclear preparations of leaves were detected using high-throughput liquid chromatography-mass spectrometry method. Many these have predicted localization signals, homology transcription factors and other regulatory proteins, are phosphorylated. Statistics summed spectral counts revealed sets with differential accumulation changes between plants. These protein previously reported expression very little overlap was found. Thus, it appears numerous post-translationally affected nucleus after infection. To our knowledge, this first indication large-scale proteomic change plant Furthermore, data reveal distinct under control show regulates accumulation. regulated likely influence broader defense responses, may facilitate development plants improved resistance.