Proteomic analysis of barley cell nuclei purified by flow sorting.
作者： Beáta Petrovská , Hana Jeřábková , Ivo Chamrád , Jan Vrána , René Lenobel
DOI: 10.1159/000365311
关键词:
摘要: Many proteins are present in the nucleus; some involved with its structural and functional organization, gene expression, cell division. The plant nuclear proteome has not been well explored. Its characterization requires extraction methods which minimize both artifactual alteration of extent contamination non-nuclear proteins. conventional multi-step fractionation procedure is laborious prone to contamination. Here, we describe a single-step method based on flow sorting. allows separation G1, S G2 phase nuclei minimizes risk by Preliminary results obtained using G1 from barley root tips indicate that sorting coupled protein/peptide mass spectrometry will permit comprehensive proteome.
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