Pulsed-field electrophoresis in microlithographic arrays

摘要: Transverse pulsed-field electrophoresis of DNA has been conducted in a silicon array engineered by optical lithography and the motion individual molecules observed fluorescence microscopy. In strong fields, can be maintained highly stretched, linear conformations. When field is switched through an obtuse angle, they head off new direction led what was formerly their tail end. This backtracking gives rise to fractionation that with molecular weight. A simple prescription exists for choosing parameters obtain particular range separation. Since motions are much more uniform than those occur gel, it anticipated arrays will permit efficient traditional gel electrophoresis. Arrays suitably scaled down size may useful sequencing.