Electrophoresis of the nucleic acids
作者： Baldomero M. Olivera , Peter Baine , Norman Davidson
DOI: 10.1002/BIP.1964.360020306
关键词:
摘要: A zone electrophoresis apparatus using ultraviolet, optics has been constructed to study nucleic acids at concentration less than 0.004%. Native DNA a mobility about 15% higher denatured over range of conditions pH and ionic strength. DNA's from different sources have closely similar mobilities. molecular weight series indicates that the is constant in 2.5 × 105 1.3 108. mobilities change expected way with but fractional charge calculated by titration curves. small decrease accompanies an increase RNA's various slightly lower except for s-RNA which travels faster.
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