DNA Gel Electrophoresis

摘要: The development of gel electrophoresis as a method separating and analyzing DNA has been one the forces driving revolution in molecular biology for last 20 years. In principle, is conceptually easy to understand technically execute. practice, there are lot small details that affect accuracy reproducibility results. This chapter presents detailed description experimental methods used electrophoresis, designed guide investigator with little or no experience this technique. described here those every day author’s laboratory; additional protocols ancillary techniques may be found Sambrook et al. (1987). All discussions refer separation double-stranded molecules slab gels, using unidirectional electric fields fluorescent detection methods. pulsed field (PFGE) large (Birren Lai 1993) capillary (Righetti Gelfi 1996) detail elsewhere.