Genetic analysis of the sugarcane (Saccharum spp.) cultivar ‘LCP 85-384’. I. Linkage mapping using AFLP, SSR, and TRAP markers

摘要: Sugarcane hybrids are complex aneu-polyploids (2n = 100–130) derived from inter-specific hybridization between ancestral polyploid species, namely S. officinarum L. and spontaneum Efforts to understand the sugarcane genome have recently been enhanced through use of new molecular marker technologies. A framework genetic linkage map Louisiana’s popular cultivar LCP 85-384 was constructed using selfed progeny based on polymorphism 64 AFLP, 19 SSR 12 TRAP primer pairs. Of 1,111 polymorphic markers detected, 773 simplex (segregated in 3:1 ratio) 182 duplex (segregate 77:4 were used construct a LOD value ≥4.0 recombination threshold 0.44. The distances pairs linked coupling phase computed Kosambi mapping function. 955 markers, 718 66 assigned 108 co-segregation groups (CGs) with cumulative length 5,617 cM density 7.16 per marker. Fifty-five 116 remained unlinked. With an estimated size 12,313 for 85-384, covered approximately 45.6% genome. Forty-four CGs into 9 homo(eo)logous (HGs) information locus-specific repulsion linkages detected CGs. Meiotic behavior chromosomes cytogenetic studies analysis this study inferred existence strong preferential chromosome pairing 85-384. This marks important beginning future QTLs associated agronomic traits Louisiana breeding programs.