PARTICLE, a Triplex-Forming Long ncRNA, Regulates Locus-Specific Methylation in Response to Low-Dose Irradiation
作者： Valerie Bríd O’Leary , Saak Victor Ovsepian , Laura Garcia Carrascosa , Fabian Andreas Buske , Vanja Radulovic
DOI: 10.1016/J.CELREP.2015.03.043
关键词: Biology 、 CpG site 、 Electrophoretic mobility shift assay 、 PRC2 、 Molecular biology 、 Chromatin immunoprecipitation 、 Intercellular transport 、 RNA 、 Cell biology 、 Protein subunit 、 Methylation
摘要: Exposure to low-dose irradiation causes transiently elevated expression of the long ncRNA PARTICLE (gene PARTICLE, promoter MAT2A-antisense radiation-induced circulating lncRNA). affords both a cytosolic scaffold for tumor suppressor methionine adenosyltransferase (MAT2A) and nuclear genetic platform transcriptional repression. In situ hybridization discloses that MAT2A associate together following irradiation. Bromouridine tracing presence in exosomes indicate intercellular transport, this is supported by ex vivo data from radiotherapy-treated patients. Surface plasmon resonance indicates forms DNA-lncRNA triplex upstream CpG island. We show represses via methylation demonstrate interacts with transcription-repressive complex proteins G9a SUZ12 (subunit PRC2). The interplay implicates lncRNA communication as recruitment gene-silencing machineries through formation response
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