Numerical methods for handling uncertainty in microarray data: an example analyzing perturbed mitochondrial function in yeast.

摘要: Publisher Summary This chapter discusses methodological issues related to the processing and evaluation of numerical results from microarray experiments. It illustrates application technology study how perturbations mitochondrial function affect gene expression in yeast. The “wet” steps spotted DNA methodology including slide washing coating, polymerase chain reaction (PCR) product manipulation printing, mRNA labeling hybridization are well described. These procedures give excellent can be followed essentially without modification. A unique exception this rule concerns interval between coating slides printing. optimal obtained using as soon possible after with poly-L-lysine. current procedure is coat immediately prior printing steam treat UV irradiate them Succinic anhydride treatment may done shortly before arrays used should materials that fresh possible.