Determination of free acidic and alkaline residues of protein via moving reaction boundary titration in microdevice electrophoresis

摘要: As two important physico-chemical parameters, the acidic and alkaline residues of protein are evident significance for evaluation properties design relevant separation analysis. However, there is still no electrophoretic method used direct detection free protein. Herein, we developed concepts moving reaction boundary (MRB) MRB titration, titration theory, microdevice electrophoresis determination In was created with acid or alkali target immobilized via highly cross-linked polyacrylamide gel (PAG). It theoretically revealed that number as a function displacement in system. proof concept, seven model proteins were chosen titration. The results showed numbers detected good agreement those obtained from amino sequences NCBI database, demonstrating feasibility theory technique. general methodology has potential application inexpensive, facilitative informative structure analysis