Expression of Cyanobacterial Acyl-ACP Reductase Elevates the Triacylglycerol Level in the Red Alga Cyanidioschyzon merolae

摘要: Nitrogen starvation is known to induce the accumulation of triacylglycerol (TAG) in many microalgae, and potential use microalgae as a source biofuel has been explored. However, nitrogen also stops cellular growth. The expression cyanobacterial acyl-acyl carrier protein (ACP) reductase unicellular red alga Cyanidioschyzon merolae chloroplasts resulted an TAG, which led increase number size lipid droplets while maintaining Transcriptome metabolome analyses showed that acyl-ACP altered activities several metabolic pathways. enzymes involved fatty acid synthesis chloroplasts, such acetyl-CoA carboxylase pyruvate dehydrogenase, were up-regulated, decarboxylation mitochondria subsequent consumption by tricarboxylic (TCA) cycle down-regulated. Aldehyde oxidizes aldehydes acids, was up-regulated expresser. This activation required for droplet changes observed C. merolae, cell growth ceased case other algal species. occur upon are quite different from those caused starvation. Therefore, there should be method further increasing storage level still response