作者: Nadine Piekarski , Lennart Olsson
DOI: 10.1111/J.1525-142X.2007.00197.X
关键词: Ambystoma mexicanum 、 Cell fate determination 、 Biology 、 Immunofluorescence 、 Somite 、 Fate mapping 、 Axolotl 、 Anatomy 、 Embryo 、 Histology
摘要: The fate of single somites has not been analyzed from a comparative perspective with modern cell-marking methods. Most what we know is based on work using quail-chick chimeras. Consequently, to degree cell conserved despite the anatomical differences among vertebrates unknown. We have cranialmost somites, focus somite two, in Mexican axolotl (Ambystoma mexicanum). Somite cells were marked by injection dextran-fluorescein and detected immunofluorescence after 2 months development paraffin sections. Our data confirm extend earlier studies classical histology salamanders. show that two contributes different muscles, skeletal elements, connective tissues head cranial trunk region. Cells three migrate latero-ventrally contribute hypobranchial muscles mm. geniohyoideus rectus cervicis. provide evidence specific formation musculature ventral processes might be variable classes vertebrates. further demonstrate cucullaris dilatator laryngis, which thought branchial origin, arise somitic material manner very similar findings indicate pattern derivatives highly within tetrapods.