Different effects of ethanol on particle phagocytosis via different receptors in human monocytes.

摘要: Endocytosis of test particles by human blood monocytes (Mo) was tested in the presence ethanol (80 mM). Phagocytosis via Fc (IgG)- or C3b receptors (R) assessed an assay which IgG- coated sheep erythrocytes (E) were used as particles. Latex parallel with opsonized E. IgG-E reduced to 67 +/- 5% control (= without ethanol), while corresponding value for C3b-E 164 26% controls. latex not affected exposure (91 8% control). The receptor functions also present during assays. In this part study, Mo incubated autologous serum 15 min at 37 degrees C. After washing cells free ethanol, binding properties Fc-R C3b-R a rosette assay. Preincubation binding, attachment seemed be more effective. experiments thus indicate different effects treatment vitro on phagocytic Mo. Control revealed no direct effect