Effect of pressure on sedimentation velocity patterns

作者: Gerson Kegeles , Michael Johnson

DOI: 10.1016/0003-9861(70)90106-2

关键词: Sedimentation coefficientSchlierenDegree of polymerizationVolume (thermodynamics)InstabilityChromatographyMyosinSedimentationMechanicsPolymerizationChemistryBiophysicsBiochemistryMolecular biology

摘要: Abstract In the case of myosin, experimental ultracentrifuge studies Josephs and Harrington have provided quantitative data for degree polymerization, formation constant at 1 atm, volume polymerization. With aid these complete we been able to predict quantitatively shapes sedimentation velocity schlieren patterns under conditions, from a rectangular cell model based on countercurrent distribution analog Bethune Kegeles. These predictions confirm reliability procedures developed by obtaining parameters, but also show that there is region extreme instability ahead moving boundaries, in agreement with generalization Kegeles second-moment coefficient considerations.

参考文章(7)
Gerson Kegeles, Michael Johnson, Effects of pressure on sedimentation velocity patterns Archives of Biochemistry and Biophysics. ,vol. 141, pp. 59- 62 ,(1970) , 10.1016/0003-9861(70)90105-0
Robert Josephs, William F. Harrington, Studies on the formation and physical chemical properties of synthetic myosin filaments. Biochemistry. ,vol. 5, pp. 3474- 3487 ,(1966) , 10.1021/BI00875A013
J. L. Bethune, Gerson Kegeles, COUNTERCURRENT DISTRIBUTION OF CHEMICALLY REACTING SYSTEMS. III. ANALOGS OF MOVING BOUNDARY ELECTROPHORESIS AND SEDIMENTATION The Journal of Physical Chemistry. ,vol. 65, pp. 1761- 1764 ,(1961) , 10.1021/J100827A019
Robert. Josephs, William F. Harrington, Stability of myosin filaments Biochemistry. ,vol. 7, pp. 2834- 2847 ,(1968) , 10.1021/BI00848A020