作者: James R. Hagler
DOI: 10.1093/EE/26.5.1079
关键词: Mark and recapture 、 Immunolabeling 、 Molecular biology 、 Hippodamia convergens 、 Mark release recapture 、 Biology 、 Anatomy 、 Immunoglobulin G 、 Coccinellidae 、 Cage 、 Retention time
摘要: A field cage study was conducted that compared the retention time between a novel immunolabeling mark-release-recapture marking technique with more conventional insect marker, fluorescent dust. Commercially purchased convergent lady beetles, Hippodamia convergens Guerin-Meneville, were marked either rabbit immunoglobulin G (IgG) or chicken IgG solution. The beetles then released into separate cages, recaptured daily, and assayed by both sandwich enzyme-linked immunosorbent assay (ELISA) direct ELISA for presence of markers. 3rd group dust, cage, examined under dissecting microscope marker. Data indicate markers retained much longer than Additionally, had greater IgG. advantages using studies over current techniques are discussed.