Microfluidic PCR device

作者: Cristian Dall'oglio , Giuseppe Emanuele Spoto , Luigi Giuseppe Occhipinti , Crocifisso Marco Antonio Renna , Laurent Drazek

DOI:

关键词: Structural engineeringLayer (electronics)OptoelectronicsMain channelMicrofluidicsMaterials scienceInlet

摘要: A microfluidic device ( 1000 - 1005 ), comprising: a semiconductor body 2 ) having first side and second b opposite to one another, housing, at the side, plurality of wells 4 depth; an inlet region 30 forming entrance point for fluid be supplied wells; main channel 6 fluidically connected region, secondary channels connecting respective well, third depth. The depth is higher than depth, which in turn According aspect, further comprises cover layer 8 arranged above body, configured sealing provided with least valve hole 54 extends through overlaps, partially, channels; flexible 14 protrusion 74 extending towards overlapping, channels, being such that, when pressure applied on it, contacts enters thus isolating from another.

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