Method, microreactor and apparatus for carrying out real-time nucleic acid amplification
作者： Daniele Ricceri , Enrico Alessi
DOI:
关键词:
摘要: A method for carrying out nucleic acid amplification, includes providing a reaction chamber (31), accommodating an array (36) of probes (37) at respective locations, hybridizing to target acids; and introducing solution (50) into the wherein contains primers, capable binding acids, nucleotides, extending enzymes sample including acids. The structure primers so that hybridization temperature (T H ) is higher than annealing whereby take place in separate ranges (R , R ).
lens.org 参考文章(12)
Liang Xu, Real-time pcr microarray based on an evanescent wave biosensor ,(2005)
Ghazala Hashmi, Michael Seul, Alice Xiang Li, Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection ,(2002)
Lawrence J. Wangh, Cristina Hartshorn, J. Aquilles Sanchez, Kenneth Pierce, Arthur Reis, John Rice, Jesse Salk, Primers, probes and methods for nucleic acid amplification ,(2005)
Babak Hassibi, Arjang Hassibi, Haris Vikalo, Multiplex Q-PCR arrays ,(2007)
Paul Eder, Attila T. Lorincz, Irina Nazarenko, Ha Thai, Richard Mallonee, Brian Lowe, Detection of nucleic acids by target-specific hybrid capture method ,(2006)
Sylvain Margaine, Dieter Husar, Isabelle Alexandre, José Remacle, Real-time quantification of multiple targets on a micro-array ,(2004)
Hubert Zipper, Herwig Brunner, Jürgen Bernhagen, Frank Vitzthum, Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications Nucleic Acids Research. ,vol. 32, pp. 0- 0 ,(2004) , 10.1093/NAR/GNH101
Isabelle Alexandre, Heinz Koehn, Martin Seippel, Jose Remacle, Reaction chamber for real time pcr comprising capture probes and permitting detection of the pcr product by hybridisation without opening the pcr vessel ,(2007)
Pia Ollikka, Integrated non-homogeneous nucleic acid amplification and detection ,(2005)
Sylvain Margaine, Dieter Husar, Isabelle Alexandre, Heinz Koehn, Jose Remacle, Nathalie Zammateo, Identification and quantification of a plurality of nucleic acids in an homogeneous assay combining real-time PCR and hybridisation to an array ,(2006)