Antibody to Langerin/CD207 localizes large numbers of CD8α+ dendritic cells to the marginal zone of mouse spleen

摘要: Dendritic cells (DCs) are strategically positioned to take up antigens and initiate adaptive immunity. One DC subset expresses CD8αα in mice is specialized capture dying process for MHC class I “cross-presentation.” Because CD8+ DCs also express DEC205/CD205, which localized splenic T cell regions, it thought that restricted zones. Here, we used a new antibody Langerin/CD207, colabels isolated CD205+ DCs, immunolabel spleen sections. The mAb labeled discrete with high levels of CD11c CD8. Surprisingly most CD207+ profiles were marginal zones surrounding white pulp nodules, only smaller numbers areas, where CD205 colabeling was noted. Despite zone location, lacked identifying molecules 3 different types macrophages, proximity and, contrast poor scavengers soluble particulate substrates. After stimulation microbial agonists, Langerin expression disappeared from the at 6–12 h, but greatly expanded by 24–48 disappeared. Therefore, anti-Langerin antibodies localize majority non-T regions mouse spleen, they distinct adjacent macrophages.