Killing cells without lysis in a method for enzyme recovery from a fermentation broth

摘要: This invention provides a method for killing fungal cells without lysing in fermentation processes order to prepare the mixture processing recover or extract an extracellularly expressed enzyme from mixture. A preferred of this comprises adjusting pH less than 2.79 using mineral acid, then adding sufficient acetic acid affect substantially complete cell kill salt can be used. The organic added, adjusted desired level. Other acids used, which case is pK selected before added useful stopping growth and any micro-organism, culture such as those containing yeast, bacteria fungi particularly systems where it them.