Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant
作者： Nest McKain , Buğra Genc , Timothy J. Snelling , R. John Wallace
DOI: 10.1016/J.MIMET.2013.10.009
关键词: Cryoprotectant 、 Archaea 、 Bacteroidetes 、 Rumen 、 Bacteria 、 16S ribosomal RNA 、 Biochemistry 、 Glycerol 、 Biology 、 Firmicutes
摘要: Bacteria and archaea in frozen (-20°C) ruminal digesta were analysed by qPCR cloning/sequencing of 16S rRNA genes. Samples with without glycerol as cryoprotectant indicated a major loss Bacteroidetes unprotected samples, resulting higher proportions Firmicutes. Archaeal numbers diversity unaffected.
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