Capacitive DNA sensor for rapid and sensitive detection of whole genome human herpesvirus‐1 dsDNA in serum
作者： Cheng Cheng , Rania Oueslati , Jayne Wu , Jiangang Chen , Shigetoshi Eda
关键词: DNA–DNA hybridization 、 Dielectrophoresis 、 Chemistry 、 Nucleic acid thermodynamics 、 Human genome 、 Genome 、 Chromatography 、 Molecular biology 、 Electrophoresis 、 Detection limit 、 DNA
摘要: This work presents a rapid, highly sensitive, low-cost, and specific capacitive DNA sensor for detection of whole genome human herpesvirus-1 DNA. is capable direct with response time 30 s, it can be used to test standard buffer or serum samples. The sensing approach based on alternating current (AC) electrokinetics. By applying an inhomogeneous AC electric field electrodes, positive dielectrophoresis induced accelerate hybridization. same applied signal also directly measures the hybridization target probe surface. Experiments are conducted optimize signal, as well buffers immobilization assay sensitive specific, no herpesvirus-2 at 5 ng/mL LOD 1.0 pg/mL (6.5 copies/μL 10.7 aM) in buffer. When testing double stranded (ds) spiked samples, yields 20.0 pg/mL (129.5 0.21 femtomolar (fM)) neat serum. In this work, dsDNA, consequently performed without use enzyme amplification, which considerably simplifies operation suitable point care disease diagnosis.
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